By Gerald Litwack
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Additional info for Vitamin K
Nutr. 92, 939–945. , and Prentice, A. (2005). Effect of apolipoprotein E genotype on vitamin K status in healthy older adults from China and the UK. Br. J. Nutr. 94, 956–961. , and Irahara, M. (2006). Undercarboxylated osteocalcin concentration in postmenopausal women receiving hormone therapy daily and on alternate days. Menopause 13, 314–322. C H A P T E R T W O VKORC1 and the Vitamin K Cycle Andrea A. Garcia* and Pieter H. Reitsma* Contents 24 25 27 29 31 31 32 I. Vitamin K Cycle: Limiting Step of Carboxylation A.
1987). Dietary fat clearance in normal subjects is regulated by genetic variation in apolipoprotein E. J. Clin. Invest. 80, 1571–1577. , and Prentice, A. (2004). Vitamin K status of older individuals in northern China is superior to that of older individuals in the UK. Br. J. Nutr. 92, 939–945. , and Prentice, A. (2005). Effect of apolipoprotein E genotype on vitamin K status in healthy older adults from China and the UK. Br. J. Nutr. 94, 956–961. , and Irahara, M. (2006). Undercarboxylated osteocalcin concentration in postmenopausal women receiving hormone therapy daily and on alternate days.
This choice was made on the basis of previous findings that VKOR was probably a membrane-bound protein. Then, knockdown experiments were performed using double-stranded small interfering RNA molecules (siRNAs) for each of the 13 gene candidates in A549 cultured cells with high intrinsic VKOR activity. Knockdown of only 1 of the 13 genes resulted in significant reduction of VKOR activity. VKORC1 and the Vitamin K Cycle 27 The putative VKOR gene was cloned and overexpressed in insect cells to confirm VKOR function and warfarin sensitivity.