DNA engineering: properties and applications by Kenji Mizoguchi, Hirokazu Sakamoto

By Kenji Mizoguchi, Hirokazu Sakamoto

This e-book provides uncomplicated information regarding DNA, besides accomplished theoretical creation to DNA. It discusses fresh advancements in divalent-metal-ion inserted M-DNA advanced, which supplies upward thrust to the potential of DNA software to digital performance. additional, the ebook describes 3 examples of purposes: optical and electric fabrics, digital units comparable to bioTFT reminiscence and color-tunable light-emitting diodes, and biofuel mobile program with use of proton conduction in DNA.

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The loss of ethidium fluorescence reflects the insertion of divalent metal ion M2+ in place of the hydrogen bond between the bases of a base pair. (A) Shows the Zn ion concentration dependence, (B) demonstrates the effect of pH on the Zn ion insertion, (C) suggests a crucial role of temperature in the Zn ion insertion, and (D) represents the insertion rate of 1 mM of various metal ion species. Copyright 2008 Canadian Science Publishing or its licensors. Reproduced with permission from Ref. [16]. in M-DNA repels ethidium with positive charge from DNA, resulting in quenching of the fluorescence.

Poly[d(A-T)] (original notation, which is the same as poly(dA-dT) or poly(dA)-poly(dT) in this book) reacts very slowly, less than 10% , but 40% in poly[d(G-C)] and almost 100% in poly[d(T-G)]·poly[d(C-A)], at 10 min. 2 [27]. Fe2+ ions react with doublestranded (DS) poly(dG-dC) twice as fast as the case of DS poly(dAdT), to form Fe3+ -poly(dG-dC) and Fe3+ -poly(dA-dT), respectively. The large difference reflecting the characteristic of the bases is another supporting evidence for the insertion of a Zn2+ ion between the bases of a base pair.

13. The magnetization curve of B-DNA at 100 K in Fig. 8 × 10−4 emu/mol-bp. Very interestingly, the freezedried DNA (FD-DNA) shows a huge paramagnetism saturating at less than 1 T without hysteresis, which is almost temperature independent up to 300 K, except for the oxygen contribution below 50 K, as shown in Fig. 13 [33]. It was reported that the long-term evacuation of DNA leads to an emergence of this kind of saturating magnetization, but as small as one-tenth of that of Fig. 12 [34]. 12 Magnetization at 100 K in B-DNA and freeze-dried DNA.

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